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origene mgmt knockout kit  (OriGene)


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    OriGene origene mgmt knockout kit
    Characterization of U1210 O 6 -methylguanine DNA methyltransferase <t>(MGMT)</t> <t>knockout</t> (KO) cells. (A) Confirmation of MGMT KO in U1242 glioblastoma (GBM) cells by western blotting. (B) MGMT KO confers temozolomide (TMZ) sensitivity to U1242 cells. Wildtype U1242 cells are resistant to TMZ induced apoptosis as indicated by minimal detection of cleaved PARP (C PARP). MGMT KO U1242 cells undergo substantially more apoptosis after TMZ treatment. Cells were treated with 20 μM TMZ for 72 h and subjected to western blotting. (C) Wildtype and MGMT KO U1242 cells exhibit nearly identical proliferation rates. Growth curves of MGMT WT and MGMT KO cells were performed in vitro .
    Origene Mgmt Knockout Kit, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/origene+mgmt+knockout+kit/MGMT+Human+Gene+Knockout+Kit/pmc12056744-42-17-17
    Average 93 stars, based on 1 article reviews
    origene mgmt knockout kit - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "O 6 -methylguanine DNA methyltransferase (MGMT) expression in U1242 glioblastoma cells enhances in vitro clonogenicity, tumor implantation in vivo , and sensitivity to alisertib-carboplatin combination treatment"

    Article Title: O 6 -methylguanine DNA methyltransferase (MGMT) expression in U1242 glioblastoma cells enhances in vitro clonogenicity, tumor implantation in vivo , and sensitivity to alisertib-carboplatin combination treatment

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2025.1552015

    Characterization of U1210 O 6 -methylguanine DNA methyltransferase (MGMT) knockout (KO) cells. (A) Confirmation of MGMT KO in U1242 glioblastoma (GBM) cells by western blotting. (B) MGMT KO confers temozolomide (TMZ) sensitivity to U1242 cells. Wildtype U1242 cells are resistant to TMZ induced apoptosis as indicated by minimal detection of cleaved PARP (C PARP). MGMT KO U1242 cells undergo substantially more apoptosis after TMZ treatment. Cells were treated with 20 μM TMZ for 72 h and subjected to western blotting. (C) Wildtype and MGMT KO U1242 cells exhibit nearly identical proliferation rates. Growth curves of MGMT WT and MGMT KO cells were performed in vitro .
    Figure Legend Snippet: Characterization of U1210 O 6 -methylguanine DNA methyltransferase (MGMT) knockout (KO) cells. (A) Confirmation of MGMT KO in U1242 glioblastoma (GBM) cells by western blotting. (B) MGMT KO confers temozolomide (TMZ) sensitivity to U1242 cells. Wildtype U1242 cells are resistant to TMZ induced apoptosis as indicated by minimal detection of cleaved PARP (C PARP). MGMT KO U1242 cells undergo substantially more apoptosis after TMZ treatment. Cells were treated with 20 μM TMZ for 72 h and subjected to western blotting. (C) Wildtype and MGMT KO U1242 cells exhibit nearly identical proliferation rates. Growth curves of MGMT WT and MGMT KO cells were performed in vitro .

    Techniques Used: Knock-Out, Western Blot, In Vitro

    The effect of O 6 -methylguanine DNA methyltransferase (MGMT) expression on 3D growth of U1242 cells. (A) Representative photomicrographs of U1242 MGMT wildtype (WT) and knockout (KO) cell growth in soft agar on days 1, 4, 7, and 10 (scale bars represent 50 μm). (B) Representative photomicrographs of U1242 MGMT WT cells, transfected with mock or MGMT siRNA, in soft agar on days 1, 4, 7, and 10 (scale bars represent 50 μm). (C) Quantification of colony sizes of MGMT WT and KO cells. (D) Quantification of mock or MGMT siRNA colony sizes transfected U1242 MGMT WT cells. (E) Confirmation of MGMT knockdown on days 1 and 4 in U1242 cells.
    Figure Legend Snippet: The effect of O 6 -methylguanine DNA methyltransferase (MGMT) expression on 3D growth of U1242 cells. (A) Representative photomicrographs of U1242 MGMT wildtype (WT) and knockout (KO) cell growth in soft agar on days 1, 4, 7, and 10 (scale bars represent 50 μm). (B) Representative photomicrographs of U1242 MGMT WT cells, transfected with mock or MGMT siRNA, in soft agar on days 1, 4, 7, and 10 (scale bars represent 50 μm). (C) Quantification of colony sizes of MGMT WT and KO cells. (D) Quantification of mock or MGMT siRNA colony sizes transfected U1242 MGMT WT cells. (E) Confirmation of MGMT knockdown on days 1 and 4 in U1242 cells.

    Techniques Used: Expressing, Knock-Out, Transfection, Knockdown

    Comparison of in vivo growth of U1242 O 6 -methylguanine DNA methyltransferase (MGMT) wildtype (WT) and knockout (KO) cells. (A) Cell lines and implanted cell numbers for each experimental group. (B) Representative H&E staining of mouse brains; (i): MGMT WT 20X, (ii): MGMT WT 50X, (iii): MGMT KO 20X, (iv): MGMT KO 50X ( n = 3 for each group, scale bars represent 200 μm).
    Figure Legend Snippet: Comparison of in vivo growth of U1242 O 6 -methylguanine DNA methyltransferase (MGMT) wildtype (WT) and knockout (KO) cells. (A) Cell lines and implanted cell numbers for each experimental group. (B) Representative H&E staining of mouse brains; (i): MGMT WT 20X, (ii): MGMT WT 50X, (iii): MGMT KO 20X, (iv): MGMT KO 50X ( n = 3 for each group, scale bars represent 200 μm).

    Techniques Used: Comparison, In Vivo, Knock-Out, Staining

    Effect of alisertib and/or carboplatin treatment on (A) O 6 -methylguanine DNA methyltransferase (MGMT) wildtype (WT) and knockout (KO) cells, and (B) Empty or MGMT overexpression vector transfected MGMT KO cell line (Each data point represents the mean value of three replicates from an independent experiment, n = 3, two-way ANOVA). (C) Kaplan-Meier survival curve of orthotopic xenograft U1242 MGMT WT cells implanted mice (vehicle, n = 5; alisertib only, n = 7; carboplatin only, n = 6; and alisertib + carboplatin, n = 7).
    Figure Legend Snippet: Effect of alisertib and/or carboplatin treatment on (A) O 6 -methylguanine DNA methyltransferase (MGMT) wildtype (WT) and knockout (KO) cells, and (B) Empty or MGMT overexpression vector transfected MGMT KO cell line (Each data point represents the mean value of three replicates from an independent experiment, n = 3, two-way ANOVA). (C) Kaplan-Meier survival curve of orthotopic xenograft U1242 MGMT WT cells implanted mice (vehicle, n = 5; alisertib only, n = 7; carboplatin only, n = 6; and alisertib + carboplatin, n = 7).

    Techniques Used: Knock-Out, Over Expression, Plasmid Preparation, Transfection

    Changes in DNA damage markers in U1242 O 6 -methylguanine DNA methyltransferase (MGMT) wildtype (WT) and knockout (KO) cells with alisertib and carboplatin treatment. (A) Representative western blots of DNA damage response markers in MGMT WT and MGMT KO cells treated with 0 to 10 μM alisertib, carboplatin, or alisertib + carboplatin. Quantification of (B) pATM, (C) pBRCA1, (D) pChk1, (E) . pChk2, and (F) pHistone-H2AX protein levels. Each data point represents the values from independent experiments. n = 3, two-way ANOVA.
    Figure Legend Snippet: Changes in DNA damage markers in U1242 O 6 -methylguanine DNA methyltransferase (MGMT) wildtype (WT) and knockout (KO) cells with alisertib and carboplatin treatment. (A) Representative western blots of DNA damage response markers in MGMT WT and MGMT KO cells treated with 0 to 10 μM alisertib, carboplatin, or alisertib + carboplatin. Quantification of (B) pATM, (C) pBRCA1, (D) pChk1, (E) . pChk2, and (F) pHistone-H2AX protein levels. Each data point represents the values from independent experiments. n = 3, two-way ANOVA.

    Techniques Used: Knock-Out, Western Blot

    Related Articles

    Knock-Out:

    Article Title: O 6 -methylguanine DNA methyltransferase (MGMT) expression in U1242 glioblastoma cells enhances in vitro clonogenicity, tumor implantation in vivo , and sensitivity to alisertib-carboplatin combination treatment
    Article Snippet: Samples and controls were loaded onto the RotorGene thermocycler (QIAGEN), gain optimization settings adjusted, and the thermal cycling program was initiated. .. Two separate guide RNAs (gRNAs) that target exon 2 of the MGMT gene are provided by the Origene MGMT Knockout Kit ( KN201612 ). ..



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    origene mgmt knockout kit  (OriGene)
    93
    OriGene origene mgmt knockout kit
    Characterization of U1210 O 6 -methylguanine DNA methyltransferase <t>(MGMT)</t> <t>knockout</t> (KO) cells. (A) Confirmation of MGMT KO in U1242 glioblastoma (GBM) cells by western blotting. (B) MGMT KO confers temozolomide (TMZ) sensitivity to U1242 cells. Wildtype U1242 cells are resistant to TMZ induced apoptosis as indicated by minimal detection of cleaved PARP (C PARP). MGMT KO U1242 cells undergo substantially more apoptosis after TMZ treatment. Cells were treated with 20 μM TMZ for 72 h and subjected to western blotting. (C) Wildtype and MGMT KO U1242 cells exhibit nearly identical proliferation rates. Growth curves of MGMT WT and MGMT KO cells were performed in vitro .
    Origene Mgmt Knockout Kit, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/origene+mgmt+knockout+kit/MGMT+Human+Gene+Knockout+Kit/pmc12056744-42-17-17
    Average 93 stars, based on 1 article reviews
    origene mgmt knockout kit - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Characterization of U1210 O 6 -methylguanine DNA methyltransferase (MGMT) knockout (KO) cells. (A) Confirmation of MGMT KO in U1242 glioblastoma (GBM) cells by western blotting. (B) MGMT KO confers temozolomide (TMZ) sensitivity to U1242 cells. Wildtype U1242 cells are resistant to TMZ induced apoptosis as indicated by minimal detection of cleaved PARP (C PARP). MGMT KO U1242 cells undergo substantially more apoptosis after TMZ treatment. Cells were treated with 20 μM TMZ for 72 h and subjected to western blotting. (C) Wildtype and MGMT KO U1242 cells exhibit nearly identical proliferation rates. Growth curves of MGMT WT and MGMT KO cells were performed in vitro .

    Journal: Frontiers in Cellular Neuroscience

    Article Title: O 6 -methylguanine DNA methyltransferase (MGMT) expression in U1242 glioblastoma cells enhances in vitro clonogenicity, tumor implantation in vivo , and sensitivity to alisertib-carboplatin combination treatment

    doi: 10.3389/fncel.2025.1552015

    Figure Lengend Snippet: Characterization of U1210 O 6 -methylguanine DNA methyltransferase (MGMT) knockout (KO) cells. (A) Confirmation of MGMT KO in U1242 glioblastoma (GBM) cells by western blotting. (B) MGMT KO confers temozolomide (TMZ) sensitivity to U1242 cells. Wildtype U1242 cells are resistant to TMZ induced apoptosis as indicated by minimal detection of cleaved PARP (C PARP). MGMT KO U1242 cells undergo substantially more apoptosis after TMZ treatment. Cells were treated with 20 μM TMZ for 72 h and subjected to western blotting. (C) Wildtype and MGMT KO U1242 cells exhibit nearly identical proliferation rates. Growth curves of MGMT WT and MGMT KO cells were performed in vitro .

    Article Snippet: Two separate guide RNAs (gRNAs) that target exon 2 of the MGMT gene are provided by the Origene MGMT Knockout Kit ( KN201612 ).

    Techniques: Knock-Out, Western Blot, In Vitro

    The effect of O 6 -methylguanine DNA methyltransferase (MGMT) expression on 3D growth of U1242 cells. (A) Representative photomicrographs of U1242 MGMT wildtype (WT) and knockout (KO) cell growth in soft agar on days 1, 4, 7, and 10 (scale bars represent 50 μm). (B) Representative photomicrographs of U1242 MGMT WT cells, transfected with mock or MGMT siRNA, in soft agar on days 1, 4, 7, and 10 (scale bars represent 50 μm). (C) Quantification of colony sizes of MGMT WT and KO cells. (D) Quantification of mock or MGMT siRNA colony sizes transfected U1242 MGMT WT cells. (E) Confirmation of MGMT knockdown on days 1 and 4 in U1242 cells.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: O 6 -methylguanine DNA methyltransferase (MGMT) expression in U1242 glioblastoma cells enhances in vitro clonogenicity, tumor implantation in vivo , and sensitivity to alisertib-carboplatin combination treatment

    doi: 10.3389/fncel.2025.1552015

    Figure Lengend Snippet: The effect of O 6 -methylguanine DNA methyltransferase (MGMT) expression on 3D growth of U1242 cells. (A) Representative photomicrographs of U1242 MGMT wildtype (WT) and knockout (KO) cell growth in soft agar on days 1, 4, 7, and 10 (scale bars represent 50 μm). (B) Representative photomicrographs of U1242 MGMT WT cells, transfected with mock or MGMT siRNA, in soft agar on days 1, 4, 7, and 10 (scale bars represent 50 μm). (C) Quantification of colony sizes of MGMT WT and KO cells. (D) Quantification of mock or MGMT siRNA colony sizes transfected U1242 MGMT WT cells. (E) Confirmation of MGMT knockdown on days 1 and 4 in U1242 cells.

    Article Snippet: Two separate guide RNAs (gRNAs) that target exon 2 of the MGMT gene are provided by the Origene MGMT Knockout Kit ( KN201612 ).

    Techniques: Expressing, Knock-Out, Transfection, Knockdown

    Comparison of in vivo growth of U1242 O 6 -methylguanine DNA methyltransferase (MGMT) wildtype (WT) and knockout (KO) cells. (A) Cell lines and implanted cell numbers for each experimental group. (B) Representative H&E staining of mouse brains; (i): MGMT WT 20X, (ii): MGMT WT 50X, (iii): MGMT KO 20X, (iv): MGMT KO 50X ( n = 3 for each group, scale bars represent 200 μm).

    Journal: Frontiers in Cellular Neuroscience

    Article Title: O 6 -methylguanine DNA methyltransferase (MGMT) expression in U1242 glioblastoma cells enhances in vitro clonogenicity, tumor implantation in vivo , and sensitivity to alisertib-carboplatin combination treatment

    doi: 10.3389/fncel.2025.1552015

    Figure Lengend Snippet: Comparison of in vivo growth of U1242 O 6 -methylguanine DNA methyltransferase (MGMT) wildtype (WT) and knockout (KO) cells. (A) Cell lines and implanted cell numbers for each experimental group. (B) Representative H&E staining of mouse brains; (i): MGMT WT 20X, (ii): MGMT WT 50X, (iii): MGMT KO 20X, (iv): MGMT KO 50X ( n = 3 for each group, scale bars represent 200 μm).

    Article Snippet: Two separate guide RNAs (gRNAs) that target exon 2 of the MGMT gene are provided by the Origene MGMT Knockout Kit ( KN201612 ).

    Techniques: Comparison, In Vivo, Knock-Out, Staining

    Effect of alisertib and/or carboplatin treatment on (A) O 6 -methylguanine DNA methyltransferase (MGMT) wildtype (WT) and knockout (KO) cells, and (B) Empty or MGMT overexpression vector transfected MGMT KO cell line (Each data point represents the mean value of three replicates from an independent experiment, n = 3, two-way ANOVA). (C) Kaplan-Meier survival curve of orthotopic xenograft U1242 MGMT WT cells implanted mice (vehicle, n = 5; alisertib only, n = 7; carboplatin only, n = 6; and alisertib + carboplatin, n = 7).

    Journal: Frontiers in Cellular Neuroscience

    Article Title: O 6 -methylguanine DNA methyltransferase (MGMT) expression in U1242 glioblastoma cells enhances in vitro clonogenicity, tumor implantation in vivo , and sensitivity to alisertib-carboplatin combination treatment

    doi: 10.3389/fncel.2025.1552015

    Figure Lengend Snippet: Effect of alisertib and/or carboplatin treatment on (A) O 6 -methylguanine DNA methyltransferase (MGMT) wildtype (WT) and knockout (KO) cells, and (B) Empty or MGMT overexpression vector transfected MGMT KO cell line (Each data point represents the mean value of three replicates from an independent experiment, n = 3, two-way ANOVA). (C) Kaplan-Meier survival curve of orthotopic xenograft U1242 MGMT WT cells implanted mice (vehicle, n = 5; alisertib only, n = 7; carboplatin only, n = 6; and alisertib + carboplatin, n = 7).

    Article Snippet: Two separate guide RNAs (gRNAs) that target exon 2 of the MGMT gene are provided by the Origene MGMT Knockout Kit ( KN201612 ).

    Techniques: Knock-Out, Over Expression, Plasmid Preparation, Transfection

    Changes in DNA damage markers in U1242 O 6 -methylguanine DNA methyltransferase (MGMT) wildtype (WT) and knockout (KO) cells with alisertib and carboplatin treatment. (A) Representative western blots of DNA damage response markers in MGMT WT and MGMT KO cells treated with 0 to 10 μM alisertib, carboplatin, or alisertib + carboplatin. Quantification of (B) pATM, (C) pBRCA1, (D) pChk1, (E) . pChk2, and (F) pHistone-H2AX protein levels. Each data point represents the values from independent experiments. n = 3, two-way ANOVA.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: O 6 -methylguanine DNA methyltransferase (MGMT) expression in U1242 glioblastoma cells enhances in vitro clonogenicity, tumor implantation in vivo , and sensitivity to alisertib-carboplatin combination treatment

    doi: 10.3389/fncel.2025.1552015

    Figure Lengend Snippet: Changes in DNA damage markers in U1242 O 6 -methylguanine DNA methyltransferase (MGMT) wildtype (WT) and knockout (KO) cells with alisertib and carboplatin treatment. (A) Representative western blots of DNA damage response markers in MGMT WT and MGMT KO cells treated with 0 to 10 μM alisertib, carboplatin, or alisertib + carboplatin. Quantification of (B) pATM, (C) pBRCA1, (D) pChk1, (E) . pChk2, and (F) pHistone-H2AX protein levels. Each data point represents the values from independent experiments. n = 3, two-way ANOVA.

    Article Snippet: Two separate guide RNAs (gRNAs) that target exon 2 of the MGMT gene are provided by the Origene MGMT Knockout Kit ( KN201612 ).

    Techniques: Knock-Out, Western Blot